Blast2GO Blog


How to perform a Fisher's Exact Test with Blast2GO

Blast2GO allows performing enrichment analysis with a Fisher's Exact Test. It is a statistical test based on contingency tables to study the significance of the association between two kind of classifications. 

Blast2GO allows to apply this test to Gene Ontology functional annotations and to compare different gene lists against each other. This is used to identify statistically significant and enrichment biological functions.

We can, for example, compare the functional annotations of a group of genes against the rest of the genome or compare the functional profile of two experimental conditions against each other. 

This video shows how to create the necessary files and how to run a Fisher's Exact Test. We will also show different ways of filtering and visualizing the results like e.g. enriched graphs, word clouds or bar charts. 


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