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Using BWA for DNA and RNA Alignment in OmicsBox

The Burrows-Wheeler Alignment algorithm (BWA) is a tool designed to align short DNA and RNA reads. BWA is based on the Burrows-Wheeler Transform (BWT), an algorithm designed to rearrange sequences into runs of similar characters to improve data compression and efficient sequence alignment in Bioinformatics. BWA supports both base space reads, e.g. from Illumina sequencing machines, and color space reads

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Differences Between GTF and GFF Files in Genomic Data Analysis

In the field of bioinformatics and genomic research, data representation is a critical aspect that influences the ease and accuracy of analysis. Two common file formats used in genomic data representation are Gene Transfer Format (GTF) and General Feature Format (GFF). While they may seem similar at a glance, there are distinct differences between these two formats that are crucial

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Tips to manage my OmicsBox subscription users

This blog post is dedicated to OmicsBox subscription administrators seeking seamless management of their subscription users. In this quick guide, we will walk you through the various options for adding new users to your subscription and provide step-by-step instructions for managing them through your BioBam account. With OmicsBox, you can update and oversee your subscription online, and as an administrator,

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BioBam Achieves ISO 27001 Recertification

In recent years, we have been updating our ISO 27001 certification status since its initial achievement in 2020. Since then, the BioBam team has been dedicated to maintaining high standards of quality and security during each annual review. We are pleased to announce that BioBam has now been recertified as a secure and responsible organization by the ISO 27001 standard.

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BioBam Trip to PAG in San Diego

  The BioBam team has recently returned from the 31st International Plant and Animal Genome Conference (PAG) in sunny California. Once again, PAG proved to be a great place for sharing, learning, and presenting the latest features of our bioinformatics solutions.   We showed OmicsBox to many attendees and hopefully improved their understanding of its rich set of features and

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Gene Set Analysis of GWAS data with MAGMA

The pursuit of genetic advancement in agricultural crops is a nuanced and ongoing scientific endeavor. The advent of Genome-Wide Association Studies (GWAS) marked a significant stride, offering a window into the intricate interplay between genetic variants and phenotypes. Yet, the scope of insights garnered through GWAS can be further enriched when coupled with refined analytical tools. The Gene Set Analysis

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Long-Read Sequence Alignment with Minimap2

  Sequence alignment is an essential element of genomics research, playing a significant role in aiding scientists in deciphering the intricacies of genetic data. When confronted with the task of aligning long reads generated by Pacific Biosciences (PacBio) or Oxford Nanopore Technologies (ONT) with precision and efficiency, scientists commonly opt for Minimap2 as their preferred tool (it is the most

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STARsolo Single-cell Quantification in OmicsBox

  What is scRNA-Seq Quantification? The first step for any Single-cell RNA-Seq analysis is to perform the quantification or, in other words, to obtain the gene expression values by cell. It is the input data for further analysis like Clustering, Trajectory, Cell-Cell Communication, Differential Expression, etc. Briefly, in order to obtain the expression values the sequencing reads are aligned to

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cutadapt omicsbox

Demultiplexing with Cutadapt in OmicsBox

Barcoding and demultiplexing in high-throughput sequencing experiments Barcoding, or indexing, is a widely used strategy in high-throughput sequencing experiments. This method enables the multiplexing of numerous samples in a single sequencing run by adding a unique DNA sequence to each sample before sequencing. During sequencing, the barcodes are read along with the DNA fragments, allowing the identification and split of

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