A complete framework for functional annotation and analysis


What is Blast2GO?

Blast2GO is a bioinformatics platform for high-quality functional annotation and analysis of genomic datasets. It allows analyzing and visualizing newly sequenced genomes by combining state-of-the-art methodologies, standard resources and algorithms. Blast2GO allows to gain biological insights fast and easy even for completely novel genomes. Perform out-of-the-box the entire workflow of functional annotation of your transcriptomic datasets including its analysis and biological interpretation.

Blast2GO is an ALL in ONE bioinformatics solution for functional annotation of (novel) sequences and the analysis of annotation data. Its main function is to assign information about the biological function of gene or protein sequences by making use of diverse public resources like comparison algorithms and databases. The software identifies already characterized similar sequences, and transfers its functional labels to the uncharacterized sequences. In this manner, it is possible to obtain functional information for a whole dataset much faster than through experimentation. Apart from mere "in-silico" functional sequence characterization, the software suite has many other functions including joined data-visualization and statistical analysis procedures. These features help the molecular biologist in the process of functional interpretation of experimental datasets. The software has been developed in JAVA, is platform independent. It basic version is offered entirely FREE to the scientific community (non-profit only).


Blast2GO Features

  • Easy to install and maintain: no informatics knowledge required.
  • Graphical and intuitive user interface.
  • Sequence annotation data presented in spread-sheet format.
  • Handle tens of thousand sequences in one project.
  • Functional annotation in 3 steps: BLAST to find homologus sequences, MAPPING to retrieve GO terms and ANNOTATION to select reliable functions.
  • Different annotation databases are supported: GO, Enzyme Codes, InterPro and KEGG.
  • Highly configurable annotation settings which adapt to your data and requirementes.
  • Statistical charts to monitor your annotation progress and all the aspects of your results.
  • Graphical display of annotation data through Gene Ontology graphs, pie and bar charts.
  • Selection features based on keywords and functional information.
  • Function enrichment analysis.
  • Tools to manipulate Gene Ontology Graphs.
  • Many input and export formats.


Blast2GO PRO

Blast2GO PRO is a subscription-based service that gives you access to advanced Blast2GO features.
By getting a Blast2GO PRO account you will be granted with a personal login to activate all PRO functions, thereby enjoying an enhanced application use within your trusted Blast2GO environment.

Key features of Blast2GO PRO:

  • Faster database connectivity.
    PRO provides a faster connection arquitecture and near-by database servers to speed up your annotation jobs.
    Simply connect to the fastest and most up-to-date Blast2GO servers via your PRO-Account.

  • Get access to CloudBlast.
    CloudBlast allows you to execute standard NCBI Blast+ sequence alignments directly via Blast2GO PRO in our dedicated computing cloud. This Blast2GO PRO Communtiy Resource is a high-performance, secure and cost-optimized soloution for your analysis. 
  • Priority support service.
    By becoming a PRO user you will have access to a support ticket system that guarantees assistance within 48 hours.
    You will also obtain a prioritized service for new feature development.
  • Many additional application features.
    PRO offers an array of interesting additional features and an accelerated feature development.
    New features allow you to work with standard sequence IDs, auto-save your projects, validate your annotations, more stastic charts and many more.


Basic and PRO Feature Comparison Chart

Features BASIC PRO
Blast2GOs 3-Step annotation procedure to generate high-quality functional labels   % %
Graphical Gene Ontology data mining  % %
Perform Enrichment Analysis % %
Basic statistics and charts for all major analysis steps % %
Work with multiple datasets in paralell  X %
Select subsets of sequences based on its biological functions, names, etc.  X %
Many different Blast options (CloudBlast, AWS, Local) X %
Fast Blast2GO PRO GO Mapping X %
Run RFAM analysis  X %
More Annotation features (taxa filter, validation, etc.)  X %
Advanced statistical charts to summarize your data analysis results (WordCloud, TreeMaps, etc.) X %
Advanced Graph Visualization (Color Graphs, Export SVG/PDF, etc.) X %
Use a local Blast2GO database installation X %
Seq. Toolbox (batch rename, find duplicates, etc.)  X %
More data-export options (customizable fasta, annot and generic data formats, fasta extract, ...) X %
Sequence and annotation import via BioMart (Verteb., Metazoa, Plants, Protists, Fungi) X %
  • blast
  • mapping
  • annot
  • interpro
  • goslim
  • fisher
  • graph
  • graph dialog
  • manual annot
  • select color
  • select
  • biomart
  • proxy
  • Perform a BLAST search with Blast2GO against a local database or via the NCBI www-BLAST.
  • View several statistics after the GO-Mapping step. (GO evidence code distributions, GO source databases, etc.)
  • After the annotation is finished sequences turn blue.
  • Improve your annotations results including protein domains and families via EBI's InterProScan.
  • Summarize a dataset via GO-Slim and perform pie charts to visualize the functional contents.
  • Perform GO Enrichment Analysis (Fisher's Exact Test) with Blast2GO.
  • Summarize the functional content of a dataset via the "Combiend Graphs" in Blast2GO.
  • Select how to highlight important areas in a GO function graph and define the information to be shown in each GO term node. Filter nodes based on different criteria.
  • Modify sequence annotations, names and description manually ...
  • Select sequences depending on their status color ...
  • Make complex sequence selection based on functional criteria like e.g. GO terms, domains etc.
  • Import functional GO information via BioMart into Blast2GO.
  • Proxy setting, monitor your Java memory consumption, etc ...


Join our Google Group


Please have a look at the FAQs, browse the Google Group, or contact us via support@blast2go.com


Read here to learn more about the scientific impact of Blast2GO: Publications, Citations and Testimonials.