Blast2GO - Features

FEATURES

Features of Blast2GO^®

Blast2GO is an ALL in ONE tool for functional annotation of (novel) sequences and the analysis of annotation data. Main Application Features are:

Easy to install and maintain: no informatics knowledge required.
Graphical and intuitive user interface.
Sequence annotation data presented in spread-sheet format.
Handle tens of thousand sequences in one project.
Functional annotation in 3 steps: BLAST to find homologus sequences, MAPPING to retrieve GO terms and ANNOTATION to select reliable functions.
Different annotation databases are supported: GO, Enzyme Codes, InterPro and KEGG.
Configurable annotation settings to adapt to your data.
Many input and export formats.
Statistical charts to monitor your annotation progress.
Graphical display of annotation data through GO graphs, pie and bar charts.
Selection features based on keywords and functional information
Function enrichment analysis.
Tools to manipulate GO graphs.
GUI-free version to integrate in programatic analysis pipe-lines.
Possibility to run annotation locally.

features

Become a PRO
Speed-up your analysis, enjoy priority support and use
advanced features!

Screenshots

Perform a BLAST search with Blast2GO against a local database or via the NCBI www-BLAST.

View several statistics after the GO-Mapping step. (GO evidence code distributions, GO source databases, etc.)

After the annotation is finished sequences turn blue.

Review annotations by viewing the mapping and annotation result in a GO-Graph.

Summarize the functional content of a dataset via the "Combiend Graphs" in Blast2GO.

Perform GO Enrichment Analysis (Fisher's Exact Test) with Blast2GO.

Review, summarize and filter the results of an Enrichment Analysis via the "Enriched GO-Graphs".

Summarize a dataset via GOSLIM and perform pie charts to visualize the functional contents.

Improve your annotations results including protein domains and families via EBI's InterProScan.

Improve your annotations results including protein domains and families via EBI's InterProScan.

Find and visualize the most interesting metabolic pathways for a group of proteins within your dataset.

Import functional GO information via BioMart into Blast2GO.

Proxy setting, Search and Select functions, monitor your Java memory consumption, etc ...

Blast2GO PRO Plugin Screenshots

Main sequences table of the Blast2GO Plugin interface

Different types of results of an Enrichment Analysis (Fisher's Exact Test)

Various charts which shows details about the annotation process with Blast2GO PRO

A basic Blast2GO annotation workflow created with the CLC bio Workbench Workflow Tool

Detailed workbench help-pages of the plugin

A "Combined Graph" shows the functional information of various or many sequences combined together in a single Gene Ontology graph. Different modes of colouring and filtering can be applied